Zika Virus IgG/IgM Test utilizes the principle of Immuno-chromatography. As the test sample flows through the membrane within the test device, the colored–Zika specific recombinant antigen-colloidal gold conjugate complexes with specific antibodies (IgM and/or IgG) of Zika virus, if present in the sample. This complex moves further on the membrane to the test region where it is captured by the anti-human IgM and/or human IgG antibodies coated on the membrane leading to formation of a colored band, which indicates a positive test results. Absence of this colored band in the test window indicates a negative test result. A built-in control line will always appear in the test window when the test has performed properly, regardless of the presence or absence of anti-Zika virus antibodies in the specimen.